IL-17F Polyclonal Antibody

$Tissue\/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;\r\nAntigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37\u2103 for 20 min;\r\nIncubation: Anti-IL-17F Polyclonal Antibody, Unconjugated(bs-10267R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining$

Tissue\/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;\r\nAntigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37\u2103 for 20 min;\r\nIncubation: Anti-IL-17F Polyclonal Antibody, Unconjugated(bs-10267R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human

Overview
Catalog #	bs-10267R
Product Name	IL-17F Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IL-17F
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	112744
Swiss Prot	Q96PD4
Subcellular location	Secreted
Synonyms	ML1; ML-1; CANDF6; IL-17F; Interleukin-17F; Cytokine ML-1; IL17F
Background	Stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF