ZCWCC1 (Ser739) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat placenta labeled with Rabbit Anti phospho-ZCWCC1 (Ser739) Polyclonal Antibody, Unconjugated (bs-10016R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Antigen: bs-10016P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-10016P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Lane 1: MCF-7 cell lysates; Lane 2: K562 cell lysates; Lane 3: 293T cell lysates; Lane 4: HepG2 cell lysates probed with ZCWCC1 (Ser739) Polyclonal Antibody, Unconjugated (bs-10016R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: MCF-7 cell lysates; Lane 2: K562 cell lysates probed with ZCWCC1 (Ser739) Polyclonal Antibody, Unconjugated (bs-10016R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Mouse testis lysates probed with ZCWCC1 (Ser739) Polyclonal Antibody, Unconjugated (bs-10016R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: 293T cell lysates; Lane 2: HepG2 cell lysates probed with ZCWCC1 (Ser739) Polyclonal Antibody, Unconjugated (bs-10016R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-10016R
Product Name	ZCWCC1 (Ser739) Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human MORC2 around the phosphorylation site of Ser739
Modification Site	Ser739
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	22880
Swiss Prot	Q9Y6X9
Subcellular location	Cytoplasm, Nucleus
Synonyms	ZCW3; ZCWCC1; MORC family CW-type zinc finger protein 2; Zinc finger CW-type coiled-coil domain protein 1; MORC2; KIAA0852
Background	Exhibits a cytosolic function in lipogenesis, adipogenic differentiation, and lipid homeostasis by increasing the activity of ACLY, possibly preventing its dephosphorylation. May act as a transcriptional repressor. Down-regulates CA9 expression.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

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