phospho-heterochromatin protein 1 (pTyr24) Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-0928R-100uL |
| Product Name | phospho-heterochromatin protein 1 (pTyr24) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Fruit Fly |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from Drosophila Melanogaster HP1 around the phosphorylation site of Tyr24 |
| Modification Site | Tyr24 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 34119 |
| Synonyms | Heterochromatin protein 1; HP1; Non-histone chromosomal protein C1A9 antigen; CG8409 |
| Background | HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are requiredfor binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-heterochromatin protein 1 (pTyr24) Polyclonal Antibody (bs-0928R) validated for, and at what dilution?
bs-0928R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-0928R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-heterochromatin protein 1 (pTyr24) Polyclonal Antibody (bs-0928R)?
KLH conjugated synthetic phosphopeptide derived from Drosophila Melanogaster HP1 around the phosphorylation site of Tyr24