CD56 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Rabbit Anti CD56\/NCAM1 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Rabbit Anti CD56\/NCAM1 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CD56\/NCAM1 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CD56\/NCAM1 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse cerebral cortex lysates probed with CD56 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Jurkat cells probed with CD56 Polyclonal Antibody, unconjugated (bs-0805R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)

$Tissue\/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CD56) polyclonal Antibody, Unconjugated (bs-0805R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Tissue\/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CD56) polyclonal Antibody, Unconjugated (bs-0805R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Chicken
Rabbit
GuineaPig

Overview
Catalog #	bs-0805R
Product Name	CD56 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD56
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4684
Swiss Prot	P13591
Subcellular location	Secreted, Cell membrane
Synonyms	CD56; NCAM; MSK39; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; NCAM1
Background	This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200