MSH2 Polyclonal Antibody
ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.", "Category"=>"Primary Antibody", "Clonality"=>"Polyclonal", "Concentration"=>"1ug/ul", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human, Mouse", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bs-0758R", "Gene ID"=>"4436", "Gene ID Link"=>"Entrez Gene: 4436", "Host"=>"Rabbit", "images"=>"[{\"id\":676,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":1220,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"\",\"cross_reactive_species_id\":2,\"cross_reactive_species_note\":\"\",\"description\":\"Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti MSH2 Polyclonal Antibody, Unconjugated (bs-0758R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":3,\"disease_id\":null,\"sample_tissue_id\":9,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"1220\\/676_raw.jpg\",\"filepath_med\":\"1220\\/676_med.jpg\",\"filepath_thumb\":\"1220\\/676_thumb.jpg\",\"display_order\":0,\"submitted_by\":null,\"lock_version\":1,\"created_at\":\"2016-06-06 20:05:50\",\"updated_at\":\"2016-06-06 20:05:50\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/676\"},{\"id\":42044,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":1220,\"productconjugation_id\":null,\"application_id\":1,\"application_note\":\"\",\"dilution\":\"1:1000\",\"cross_reactive_species_id\":2,\"cross_reactive_species_note\":\"\",\"description\":\"Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH\\/3T3 cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human SW480 cell lysates probed with MSH2 Polyclonal Antibody, Unconjugated (bs-0758R) at 1:1000 dilution and 4\\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\\u02daC.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":3,\"disease_id\":null,\"sample_tissue_id\":39,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"1220\\/42044_raw.jpg\",\"filepath_med\":\"1220\\/42044_med.jpg\",\"filepath_thumb\":\"1220\\/42044_thumb.jpg\",\"display_order\":2,\"submitted_by\":null,\"lock_version\":1,\"created_at\":\"2022-08-27 04:49:37\",\"updated_at\":\"2022-08-27 04:49:37\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/42044\"}]", "Immunogen Sequence"=>"101-200/935", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Notes"=>"AbBy Fluor Push", "Predicted Reactivity"=>"Cow", "Product URL"=>"https://www.biossusa.com/products/bs-0758R", "Purification"=>"Purified by Protein A.", "Size"=>"100uL", "Source"=>"KLH conjugated synthetic peptide derived from human MSH-2", "Storage"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.", "Storage Buffer"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.", "Storage Condition"=>"Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.", "Subcellular Locations"=>"Nucleus", "Swiss Prot"=>"P43246", "Swiss Prot Link"=>"Swiss Prot: P43246", "Synonyms"=>"FCC1; COCA1; HNPCC; LCFS2; HNPCC1; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2", "Target Protein"=>"MSH2"} -->
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0758R-100uL |
| Product Name | MSH2 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Cow |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MSH-2 |
| Immunogen Range | 101-200/935 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4436 |
| Swiss Prot | P43246 |
| Subcellular location | Nucleus |
| Synonyms | FCC1; COCA1; HNPCC; LCFS2; HNPCC1; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2 |
| Background | Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MSH2 Polyclonal Antibody (bs-0758R) validated for, and at what dilution?
bs-0758R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MSH2 Polyclonal Antibody (bs-0758R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Cow.
How should bs-0758R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MSH2 Polyclonal Antibody (bs-0758R)?
KLH conjugated synthetic peptide derived from human MSH-2 Immunogen range: 101-200/935.