RANKL/CD254 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti OPGL\/RANKL\/ODF Polyclonal Antibody, Unconjugated (bs-0747R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Human raji cells probed with RANKL Polyclonal Antibody, Unconjugated (bs-0747R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

mouse splenocytes cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RANKL Polyclonal Antibody(bs-0747R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).