PEPT1 Polyclonal Antibody

HCCLM3 cells probed with Rabbit Anti-PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$Mouse large intestine lysates probed with PEPT1 Polyclonal Antibody, unconjugated (bs-0689R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Mouse large intestine lysates probed with PEPT1 Polyclonal Antibody, unconjugated (bs-0689R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

SH-sy5y (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PEPT1 Antibody (bs-0689R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0689R
Product Name	PEPT1 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from chicken PEPT1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	378789
Swiss Prot	Q90WH8
Subcellular location	Cell membrane
Synonyms	Oligopeptide transporter, small intestine isoform; Peptide transporter 1; Intestinal H+/peptide cotransporter; Solute carrier family 15 member 1SLC15A1; +peptide cotransporter; HPECT1; HPEPT1; Human peptide transporter HPEPT1 mRNA complete cds; Intestinal H; Oligopeptide transporter small intestine isoform; PEPT1; Peptide transporter 1; Solute carrier family 15 oligopeptide transporter member 1.
Background	PEPT1(Oligopeptide transporter, small intestine isoform (Peptide transporter 1) (Intestinal H(+)/peptide cotransporter) (Solute carrier family 15 member 1) Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides. May constitute a major route for the absorption of protein digestion end-products. Subcellular Location__membrane; Multi-pass membrane protein. Tissue Specificity Intestine, kidney, liver and low in brain. Belongs to the PTR2/POT transporter (TC 2.A.17) family.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400