CD8 Polyclonal Antibody

Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:250.

$Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: CD8 Antibody, conjugated (bs-0648R-AF488) 1:400, 90 minutes at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: CD8 Antibody, conjugated (bs-0648R-AF488) 1:400, 90 minutes at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.

Applications

FCM
IHC-P
IF(IHC-P)
Flow-Cyt

Reactivity

Human
Mouse

Overview
Catalog #	bs-0648r
Product Name	CD8 Polyclonal Antibody
Applications	FCM, IHC-P, IF(IHC-P), Flow-Cyt
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse CD8
Immunogen Range	51-150/247
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	12525
Swiss Prot	P01731
Subcellular location	Cell membrane
Synonyms	Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Lyt2
Background	Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Application Dilution
FCM	1:50
IHC-P	1ug/Test
IF(IHC-P)	1:50-200
Flow-Cyt	1ug/Test