CD45 Polyclonal Antibody

$Raji cells probed with \\tCD45 Polyclonal Antibody, Unconjugated (bs-0522R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

Raji cells probed with \\tCD45 Polyclonal Antibody, Unconjugated (bs-0522R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bs-0522R
Product Name	CD45 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)
Specificity	The antibody is predicted to work with Mouse and Rat samples, but with decreased sensitivity compared to human samples.
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD45
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5788
Swiss Prot	P08575
Subcellular location	Cell membrane
Synonyms	LCA; LY5; B220; CD45; L-CA; T2; CD45R; GP18; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; PTPRC
Background	Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200