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Rabbit Anti-Biotin

Applications

  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Biotin
Overview
Catalog # bs-0311R
Product Name Rabbit Anti-Biotin
Applications
ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Biotin
Specifications
Conjugation Unconjugated
Host Rabbit
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Affinity purified by Protein A
Storage Buffer Lyophilized
Storage Condition Store at -20°C for 12 months.
Target
Background Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Application Dilution
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Rabbit Anti-Biotin (bs-0311R) validated for, and at what dilution?

bs-0311R is validated for ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Rabbit Anti-Biotin (bs-0311R) react with?

Tested reactivity: Biotin.

How should bs-0311R be stored?

Store at -20°C for 12 months. Storage buffer: Lyophilized

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