Rabbit Anti-Biotin
Applications
Reactivity
| Overview | |
| Catalog # | bs-0311R |
| Product Name | Rabbit Anti-Biotin |
| Applications |
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Biotin |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Affinity purified by Protein A |
| Storage Buffer | Lyophilized |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Background | Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Rabbit Anti-Biotin (bs-0311R) validated for, and at what dilution?
bs-0311R is validated for ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Rabbit Anti-Biotin (bs-0311R) react with?
Tested reactivity: Biotin.
How should bs-0311R be stored?
Store at -20°C for 12 months. Storage buffer: Lyophilized
