His tag Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-0287R-100uL |
| Product Name | His tag Polyclonal Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | synthetic peptide: HHHHHH conjugated to KLH |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Synonyms | HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
| Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is His tag Polyclonal Antibody (bs-0287R) validated for, and at what dilution?
bs-0287R is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does His tag Polyclonal Antibody (bs-0287R) react with?
Tested reactivity: Species independent.
How should bs-0287R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise His tag Polyclonal Antibody (bs-0287R)?
synthetic peptide: HHHHHH conjugated to KLH