FoxP3 Polyclonal Antibody

Name: FoxP3 Polyclonal Antibody
Brand: Bioss
SKU: bs-0269R
Price: 375.0 USD
Availability: InStock

$MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FoxP3 Polyclonal Antibody(bs-0269R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FoxP3 Polyclonal Antibody(bs-0269R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FoxP3 Polyclonal Antibody, Unconjugated (bs-0269R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with FoxP3 polyclonal antibody, unconjugated (bs-0269R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with FoxP3 polyclonal antibody, unconjugated (bs-0269R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-F
IHC-P

Reactivity

Mouse
Rat

Predicted Reactivity

Mouse

Overview
Catalog #	bs-0269R-100uL
Product Name	FoxP3 Polyclonal Antibody
Applications	WB IHC-F IHC-P
Reactivity	Mouse, Rat
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FoxP3
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	20371
Swiss Prot	Q99JB6
Subcellular location	Nucleus
Synonyms	AIID; DIETER; IPEX; JM2; PIDX; XPID; scurfin; sf; RGD1562112; FOXP3_HUMAN; FOXP3; FOXP3_MOUSE; D3ZKI1_RAT;
Background	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:500-2000
IHC-F	1:200-1000
IHC-P	1:200-1000

Frequently asked questions

Which applications is FoxP3 Polyclonal Antibody (bs-0269R) validated for, and at what dilution?

bs-0269R is validated for WB (1:500-2000); IHC-F (1:200-1000); IHC-P (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FoxP3 Polyclonal Antibody (bs-0269R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.

How should bs-0269R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FoxP3 Polyclonal Antibody (bs-0269R)?

KLH conjugated synthetic peptide derived from human FoxP3

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FoxP3 Polyclonal Antibody

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Predicted Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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