Fas Ligand Polyclonal Antibody

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Jurkat lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200 overnight at 4\u00b0C, DAB staining.

Lane 1: Mouse Bone lysates; Lane 2: Rat Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-0216R
Product Name	Fas Ligand Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Fas Ligand
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	356
Swiss Prot	P48023
Subcellular location	Nucleus, Secreted, Cell membrane, Extracellular matrix
Synonyms	APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1; Tumor necrosis factor ligand superfamily member 6; Apoptosis antigen ligand; CD95 ligand; Fas antigen ligand; Fas ligand; FASLG
Background	Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200