Estrogen Receptor alpha + beta Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0174R-100uL |
| Product Name | Estrogen Receptor alpha + beta Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Estradiol Receptor alpha + beta |
| Immunogen Range | 201-300/595 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2099, 2100 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Estrogen Receptor alpha/beta; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER Beta; ER-alpha; ER-beta; ER[a]; ER[b]; Era; ERalpha; Erb; Erb2; ERbeta; ESR; ESR BETA; ESR1; ESR1_HUMAN; ESR2; ESRA; ESRB; Estr; Estra; Estradiol Receptor alpha; Estradiol receptor; Estradiol Receptor beta; ESTRB; Estrogen receptor 1 alpha; Estrogen Receptor 1; Estrogen receptor 2 ER beta; Estrogen Receptor 2; Estrogen receptor 2 ER beta; Estrogen receptor alpha; Estrogen receptor; Estrogen receptor beta 4; Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; NR3A2; Nuclear receptor subfamily 3 group A member 1; Nuclear receptor subfamily 3 group A member 2; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR. |
| Background | The estrogen receptor (ER) is a 66 kDa protein that mediates the actions of estrogens in estrogen responsive tissues. It is a member of a large superfamily of nuclear hormone receptors that function as ligand activated transcription factors. The ER gene consists of more than 140 kb of genomic DNA divided into 8 exons. These translate into a protein with six functionally discrete domains, labeled A through F. A second form of the estrogen receptor, ER beta has recently been described. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti estrogen (e.g. tamoxifen) therapy. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Estrogen Receptor alpha + beta Antibody (bs-0174R) validated for, and at what dilution?
bs-0174R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Estrogen Receptor alpha + beta Antibody (bs-0174R) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.
How should bs-0174R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Estrogen Receptor alpha + beta Antibody (bs-0174R)?
KLH conjugated synthetic peptide derived from human Estradiol Receptor alpha + beta Immunogen range: 201-300/595.