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SPHK2 Polyclonal Antibody

Mouse stomach lysates probed with SPHK2 Polyclonal Antibody, unconjugated (bs-2653R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Mouse stomach lysates probed with SPHK2 Polyclonal Antibody, unconjugated (bs-2653R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Tissue\/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37\u00b0C for 90 minutes, DAPI (5ug\/ml, blue) was used to stain the cell nuclei.
Tissue\/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37\u00b0C for 90 minutes, DAPI (5ug\/ml, blue) was used to stain the cell nuclei.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SPHK2) polyclonal Antibody, Unconjugated (bs-2653R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SPHK2) polyclonal Antibody, Unconjugated (bs-2653R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human SH-SY5Y cell Lysates; Lane 2: Human A549 cell Lysates; Lane 3: Human HepG2 cell Lysates. Probed with SPHK2 polyclonal Antibody, unconjugated (bs-2653R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human SH-SY5Y cell Lysates; Lane 2: Human A549 cell Lysates; Lane 3: Human HepG2 cell Lysates. Probed with SPHK2 polyclonal Antibody, unconjugated (bs-2653R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
25 ug total protein per lane of various lysates (see on figure) probed with SPHK2 polyclonal antibody, unconjugated (bs-2653R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with SPHK2 polyclonal antibody, unconjugated (bs-2653R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IF
  • IHC-P

Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-2653r-100uL
Product Name SPHK2 Polyclonal Antibody
Applications
WB ELISA IHC-F IF IHC-P
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPHK2
Immunogen Range 101-200/654
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 56848
Swiss Prot Q9NRA0
Subcellular location Cytoplasm, Cell membrane
Synonyms SK 2; SK-2; SPK 2; SPK-2; Sphingosine kinase 2; SPHK2
Background Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
IHC-F 1:100-500
IF 1:100-500
IHC-P 1:100-500

Frequently asked questions

Which applications is SPHK2 Polyclonal Antibody (bs-2653R) validated for, and at what dilution?

bs-2653R is validated for WB (1:500-2000); ELISA (1:5000-10000); IHC-F (1:100-500); IF (1:100-500); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPHK2 Polyclonal Antibody (bs-2653R) react with?

Tested reactivity: Human, Mouse.

How should bs-2653R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPHK2 Polyclonal Antibody (bs-2653R)?

KLH conjugated synthetic peptide derived from human SPHK2 Immunogen range: 101-200/654.

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