Snail1/2/3 Polyclonal Antibody

Formalin-fixed and paraffin embedded human pancreas carcinoma labeled with Rabbit Anti-Snail Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Formalin-fixed and paraffin-embedded human laryngocarcinoma labeled with Rabbit Anti-Snail Polyclonal Antibody, Unconjugated(bs-1371R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Snail1\/2\/3 Polyclonal Antibody(bs-1371R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).