PBP Recombinant Antbody Kit (KD-Validated)

Flow cytometric analysis of PEBP1 expression in HeLa cells using PEBP1 antibody (1:2,000). Green, isotype control; red, PEBP1.

$Immunocytochemical staining of Hela cells with anti-PEBP1 antibody (1:1,000). Nuclei were stained blue with DAPI; PEBP1 was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 \u03bcm.$

Immunocytochemical staining of Hela cells with anti-PEBP1 antibody (1:1,000). Nuclei were stained blue with DAPI; PEBP1 was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 \u03bcm.

$Western blotting analysis using anti-PEBP1 antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PEBP1 antibody (1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.$

Western blotting analysis using anti-PEBP1 antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PEBP1 antibody (1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.

$Western blotting analysis using anti-PEBP1 antibody. PEBP1 expression in wild type (WT) and PEBP1 shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulin serves as a loading control. The blot was incubated with anti-PEBP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.$

Western blotting analysis using anti-PEBP1 antibody. PEBP1 expression in wild type (WT) and PEBP1 shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulin serves as a loading control. The blot was incubated with anti-PEBP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-90286r-50µL
Product Name	PBP Recombinant Antbody Kit (KD-Validated)
Applications	WB, FCM, IC
Specificity	Knockdown Validated
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human PEBP1
Immunogen Range	64-99/187
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Affinity Purified
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	-20 °C
Target
Gene ID	5037
Swiss Prot	P30086
Synonyms	PEBP1; Phosphatidylethanolamine Binding Protein 1; RKIP; PEBP; HCNP; PBP; Hippocampal Cholinergic Neurostimulating Peptide; Phosphatidylethanolamine-Binding Protein 1; Raf Kinase Inhibitory Protein; Raf Kinase Inhibitor Protein; Prostatic Binding Protein; Neuropolypeptide H3; HCNPpp; PEBP-1; Epididymis Secretory Protein Li 34; Epididymis Secretory Protein Li 96; Epididymis Luminal Protein 210; Prostatic-Binding Protein; HEL-S-34; HEL-S-96; HEL-210
Background	Cell Line: Hela Knockdown technology: shRNA Knockdown
Application Dilution
WB	1:300-5000
FCM	1:20-100
IC	1:1000