Chemerin Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-10410R-100uL |
| Product Name | Chemerin Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat chemerin |
| Immunogen Range | 101-163/163 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 297073 |
| Subcellular location | Secreted |
| Synonyms | Rarres2; TIG2; tazarotene induced gene 2; Retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; RAR-responsive protein TIG2; Chemerin. |
| Background | Chemerin, also known as retinoic acid receptor responder 2 (RARRES2) and tazarotene induced gene 2 (TIG2), is a chemoattractant protein for cells expressing G-protein-linked receptor chemokine-like receptor 1 (CMKLR1, ChemR23 or GPCR-DEZ). The protein is synthesized as a secreted precursor, prochemerin (18 kDa), which is poorly active. Upon proteolytic removal of the last six amino acids, prochemerin is converted into mature active chemerin (15-16 kDa) that acts as a strong and highly specific agonist of its receptor CMKLR1. Chemerin is a newly described adipokine with effects on adipocyte differnetiation and metabolism. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Chemerin Polyclonal Antibody (bs-10410R) validated for, and at what dilution?
bs-10410R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Chemerin Polyclonal Antibody (bs-10410R) react with?
Tested reactivity: Human, Mouse, Rat, Rabbit.
How should bs-10410R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Chemerin Polyclonal Antibody (bs-10410R)?
KLH conjugated synthetic peptide derived from rat chemerin Immunogen range: 101-163/163.