| Overview |
| bsm-70202M |
| Nuclei Antibody |
| IHC, ICC |
| Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections. |
| Human |
| Specifications |
| Unconjugated |
| Mouse |
| Human cell homogenate. |
| Monoclonal |
| 235-1 |
| IgG1 |
| Lot Dependent |
| Concentrated tissue culture supernatant |
| Concentrated tissue culture supernatent |
| Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. |
| Target |
| HNA antibody, Human Nuclear Antigen antibody, HuNu antibody |
| This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. |
| Application Dilution |
| IHC |
|
| ICC |
1:100-500 |
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