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Primary Antibodies

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Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs-6313P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6313R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Mouse, Rat, Others, Monkey

Conjugate: Unconjugated

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 12kD. Observed band size:48kD.\\n
Image provided by the Independent Validation Program (badge number 29759). Lane 1: HeLa cell extract, Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0052R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: Unconjugated

Catalog #1: bs-0052R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: Cy3

Catalog #1: bs-0052R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: Biotin

Catalog #1: bs-0052R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: HRP

Catalog #1: bs-0052R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: Cy5

Catalog #1: bs-0052R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: FITC

Catalog #1: bs-0052R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: Cy7

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PSD-95 Polyclonal Antibody (bs-0179R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human SH-SY5Y cell lysates probed with PSD95 Polyclonal Antibody, Unconjugated (bs-0179R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0179R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Catalog #1: bs-0179R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Biotin

Catalog #1: bs-0179R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: HRP

Catalog #1: bs-0179R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy3

Catalog #1: bs-0179R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy5

Catalog #1: bs-0179R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: FITC

Catalog #1: bs-0179R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy7

Catalog #1: bs-0215R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: HRP

Catalog #1: bs-0215R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Catalog #1: bs-0215R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy3

Catalog #1: bs-0215R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Biotin

Catalog #1: bs-0215R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: FITC

Catalog #1: bs-0215R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy7

Catalog #1: bs-0215R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy5

Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1299R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Unconjugated

Catalog #1: bs-1299R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Monkey

Conjugate: Biotin

Catalog #1: bs-1299R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Monkey

Conjugate: HRP

Catalog #1: bs-1299R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Cy5

Catalog #1: bs-1299R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Cy3

Catalog #1: bs-1299R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Cy7

Catalog #1: bs-2416R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-MAPKAPK2 (Thr222) Polyclonal Antibody, Unconjugated (bs-3261R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with MAPKAPK2 (Thr222) Polyclonal Antibody, Unconjugated (bs-3261R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse lymph node lysates; Lane 2: Mouse bone lysates probed with MAPKAPK2 (Thr222) Polyclonal Antibody, Unconjugated (bs-3261R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Lymph lysates; Lane 3: Mouse Bone lysates; Lane 4: Mouse Spleen lysates probed with MAPKAPK2 (Thr222) Polyclonal Antibody, Unconjugated (bs-3261R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3261R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Unconjugated

Catalog #1: bs-3261R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Monkey

Conjugate: Biotin

Catalog #1: bs-3261R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Monkey

Conjugate: HRP

Catalog #1: bs-3261R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Cy5

Catalog #1: bs-3398R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Monkey

Conjugate: Biotin

Catalog #1: bs-3398R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Monkey

Conjugate: Unconjugated

Catalog #1: bs-3398R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Monkey

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-TBK1 (Ser172) Polyclonal Antibody, Unconjugated(bs-3440R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human Raji cell lysates; Lane 2: Human HeLa cell lysates probed with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (bs-3440R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3440R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3567R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Monkey

Conjugate: Biotin

Catalog #1: bs-3567R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Monkey

Conjugate: HRP

Catalog #1: bs-3567R

Applications: WB, ELISA

Reactivity: Human, Mouse, Monkey

Conjugate: Unconjugated

Catalog #1: bs-3932R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Monkey

Conjugate: Unconjugated

Catalog #1: bs-3932R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat, Monkey

Conjugate: HRP

Catalog #1: bs-3932R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat, Monkey

Conjugate: Biotin

Catalog #1: bs-3932R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Monkey

Conjugate: Cy3

Catalog #1: bs-3932R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Monkey

Conjugate: Cy5

Catalog #1: bs-3932R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Monkey

Conjugate: Cy7

Catalog #1: bs-3932R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Monkey

Conjugate: FITC

Catalog #1: bs-4507R

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig, Virus, Monkey

Conjugate: Unconjugated