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Primary Antibodies

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Catalog #1: bs-5151R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy7

Catalog #1: bs-5151R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5

Catalog #1: bs-19299R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Goat

Conjugate: Unconjugated

Catalog #1: bs-19299R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Goat

Conjugate: HRP

Catalog #1: bs-19299R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Goat

Conjugate: Biotin

Catalog #1: bs-8512R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Goat

Conjugate: Biotin

Catalog #1: bs-8512R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Goat

Conjugate: HRP

Catalog #1: bs-8512R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Goat

Conjugate: Unconjugated

Catalog #1: bs-8512R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Goat

Conjugate: Cy3

Catalog #1: bs-8512R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Goat

Conjugate: Cy5

Catalog #1: bs-8512R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Goat

Conjugate: Cy7

Catalog #1: bs-8512R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Goat

Conjugate: FITC

Rat lung lysate 30ug, probed (bs-0061R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Unconjugated

Catalog #1: bs-0646R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: Cy5.5

Catalog #1: bs-2130R-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-2210R-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Cy5.5

Catalog #1: bs-0061R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Cy5.5

Catalog #1: bs-0118R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Goat

Conjugate: Cy5.5

Catalog #1: bs-0201R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Goat

Conjugate: Cy5.5

Catalog #1: bs-0279R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Cy5.5

Catalog #1: bs-0292R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat, Others, Cow

Conjugate: Cy5.5

Catalog #1: bs-0351R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: Cy5.5

Catalog #1: bs-0523R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-0814R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Sheep, Goat, Cow

Conjugate: Cy5.5

Catalog #1: bs-0923R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-0945R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-0963R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-1498R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-2736R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-3806R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: Cy5.5

Catalog #1: bs-4590R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-5151R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5.5

Catalog #1: bs-8512R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Goat

Conjugate: Cy5.5

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse placenta lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0718R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Rabbit, Goat, Others

Conjugate: Unconjugated

Lane 1: Milk (Goat) Lysate at 2 ug\r\nLane 2: Milk (Cow) Lysate at 30 ug\r\nLane 3: Milk (Cow) Lysate at 3 ug\r\nLane 4: Milk (Human) Lysate at 30 ug\r\nPrimary: Anti-Beta-casein (bs-10032R) at 1\/1000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution
Cow milk probed with Rabbit Anti-Beta-casein Polyclonal Antibody, Unconjugated (bs-10032R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.

Catalog #1: bs-10032R

Applications: WB, ELISA, IHC-F

Reactivity: Human, Bovine, Goat, Cow

Conjugate: Unconjugated

Catalog #1: bs-10032R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Human, Bovine, Goat, Cow

Conjugate: Biotin

Catalog #1: bs-10032R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Human, Bovine, Goat, Cow

Conjugate: HRP

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Transferrin Polyclonal Antibody, Unconjugated (bs-2052R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human Serum probed with Transferrin Polyclonal Antibody, Unconjugated (bs-2052R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Serum; Lane 2: Mouse Serum; Lane 3: Rat Serum; Lane 4: Goat Serum probed with Transferrin Polyclonal Antibody, Unconjugated (bs-2052R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Transferrin(Serum Loading Control) Polyclonal Antibody(bs-2052R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-2052R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-2052R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Biotin

Catalog #1: bs-2052R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Goat

Conjugate: HRP

Catalog #1: bs-6642R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-8568R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Goat

Conjugate: Unconjugated

Catalog #1: bs-23551R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Goat, Cow

Conjugate: Unconjugated

Catalog #1: bs-23551R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Goat, Cow

Conjugate: Biotin

Catalog #1: bs-23551R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Goat, Cow

Conjugate: HRP

Catalog #1: bs-24172R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Goat, Cow

Conjugate: Unconjugated

Catalog #1: bs-24172R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Goat, Cow

Conjugate: Biotin

Catalog #1: bs-24172R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Goat, Cow

Conjugate: HRP