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Primary Antibodies

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Catalog #1: bs-5447R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti phospho-IGF1R(Tyr980) Polyclonal Antibody,Unconjugated (bs-5447R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R (Tyr980) Antibody(bs-5447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R (Tyr980) Polyclonal Antibody(bs-5447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-5447R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-5447R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0444R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat colon carcinoma labeled with Anti-Slc22A17 Y Polyclonal Antibody, Unconjugated (bs-0444R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human U-2OS cell lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human Siha cell lysates probed with Slc22A17 Polyclonal Antibody, Unconjugated (bs-0444R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0444R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0444R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-13188R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-FMRP (Ser500) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse brain lysates probed with Rabbit Anti-FMRP (Ser500) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-13188R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-13188R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: HRP

Catalog #1: bs-0115R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: HRP

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Placenta lysates\uff1bLane 2: Mouse Lung lysates probed with AKT1\/2\/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (bs-0115R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0115R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Unconjugated

Catalog #1: bs-0115R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Biotin

Formalin-fixed and paraffin embedded human myometrium labeled with Anti-phospho-AKT1 (Tyr474)Polyclonal Antibody, Unconjugated (bs-10133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with AKT1\/2\/3 (Tyr474) Polyclonal Antibody, unconjugated (bs-10133R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n
Mouse lung lysates probed with AKT1\/2\/3 (Tyr474) Polyclonal Antibody, unconjugated (bs-10133R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n

Catalog #1: bs-10133R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-10133R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-10133R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0876R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: HRP

Catalog #1: bs-0876R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Biotin

Formalin-fixed and paraffin embedded human endometrium tissue labeled with Anti-phospho-AKT\/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse tumor with labeled Anti-phospho-AKT\/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-AKT(Ser473) Polyclonal Antibody, Unconjugated (bs-0876) at 1:300 overnight at 4˚C. Incubate with HRP conjugated Goat-Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000 for 90min at 37˚C.
U937 cell lysates probed with Anti-AKT1\/2\/3 (Ser472\/Ser473\/Ser474) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0876R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Unconjugated

Catalog #1: bs-4842R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-4842R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-4842R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0014R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0014R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-0014R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-4588R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-4588R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human placenta labeled with Anti-IGF I Polyclonal Antibody, Unconjugated (bs-4588R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: IGF1 Recombinat Protein lysates; Lane 2: Contro(-) lysates probed with IGF 1 Polyclonal Antibody, Unconjugated (bs-4588R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-4588R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit pancreas tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat lung lysate 30ug, probed (bs-0081R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

Catalog #1: bs-0081R

Applications: WB, ELISA, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Catalog #1: bs-0081R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: HRP

Catalog #1: bs-0081R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Biotin

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-CD146\/MCAM Polyclonal Antibody, Unconjugated (bs-1618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD146\/MCAM Polyclonal Antibody, Unconjugated (bs-1618R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Lane 1: U251 cell lysates; Lane 2: U-2os cell lysates probed with CD146 Polyclonal Antibody, Unconjugated (bs-1618R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Huvec cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U2OS cell lysates; Lane 4: Human U251 cell lysates probed with CD146 Polyclonal Antibody, Unconjugated (bs-1618R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1618R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1618R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-1618R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Lane 1:Siha cell lysates probed with MCAM(Tyr641) Polyclonal Antibody, Unconjugated (bs-5472R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human U2OS cell lysates; Lane 6: Human U87MG cell lysates probed with phospho-MCAM (Tyr641) Polyclonal Antibody, Unconjugated (bs-5472R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5472R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human stomach labeled with Anti-Inhibin beta A\/Activin A Polyclonal Antibody, Unconjugated (bs-1774R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Activin A, Unconjugated (bs-1774R) followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1774R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1774R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1774R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2636R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2636R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-2636R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded human breast cancer tissue labeled with Anti-LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human K562 cell lysates probed with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1311R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Rat splenocytes stained with Anti- TLR6\/CD286 Polyclonal Antibody, PE-CY5.5 Conjugated (bs-2716R-PE-Cy5.5) at 1:25.
Rat splenocytes stained with Anti- TLR6\/CD286 Polyclonal Antibody, PE-CY5.5 Conjugated (bs-2716R-PE-Cy5.5) at 1:25
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TLR6 Polyclonal Antibody, Unconjugated (bs-2716R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with TLR6 Polyclonal Antibody, Unconjugated (bs-2716R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-2716R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1311R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-1311R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded rat brain ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with VEGF-A Polyclonal Antibody, Unconjugated (bs-1957R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat Placenta tissue lysates probed with VEGFA Polyclonal Antibody, Unconjugated (bs-1957R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1957R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1957R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1957R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1665R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated