Human PGC Ready-To-Use IHC Kit
Human PGC Ready-To-Use IHC Kit
Cat. No.: IHC0191HSample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of pepsinogen and pepsin especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine.
Synonyms
Gastricsin, Pepsinogen C, PGC, Pepsinogen II, PEPC, PGII.
Materials Supplied
Number | Component | 50T | Concentration | Storage |
---|---|---|---|---|
1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
4 | Blocking Buffer | 3ml | RTU | 2-8°C |
5 | Primary Antibody (Human PGC Mouse mAb) | 6ml | RTU | 2-8°C |
6 | Secondary Antibody (HRP-Goat anti-Mouse IgG pAb) | 6ml | RTU | 2-8°C |
7 | Chromogen Component A | 0.3ml | RTU | -20°C |
8 | Chromogen Component B | 0.3ml | RTU | -20°C |
9 | Counter Staining Reagent | 5ml | RTU | RT |
10 | Differentiation Reagent | 6ml | RTU | RT |
11 | Mounting Media | 5ml | RTU | RT |
12 | Control slide (Human gastric fundus) | 1 slide | RTU | RT |
13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
As DAB is a carcinogen, please take necessary precautions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.