Human Lamin B Ready-To-Use IHC Kit
Human Lamin B Ready-To-Use IHC Kit
Cat. No.: IHC0183HSample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Defects in the LMNB1 gene can cause Leukodystrophy, demyelinating, autosomal dominant, adult-onset (ADLD).
Synonyms
ADLD, lamin-B1, LMN, LMN2, LMNB, LMNB1, LMB1, MGC111419, OTTHUMP00000159218.
Materials Supplied
Number | Component | 50T | Concentration | Storage |
---|---|---|---|---|
1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
4 | Blocking Buffer | 3ml | RTU | 2-8°C |
5 | Primary Antibody (Human Lamin B (Nuclear Loading Control) Mouse mAb) | 6ml | RTU | 2-8°C |
6 | Secondary Antibody (HRP-Goat anti-Mouse IgG pAb) | 6ml | RTU | 2-8°C |
7 | Chromogen Component A | 0.3ml | RTU | -20°C |
8 | Chromogen Component B | 0.3ml | RTU | -20°C |
9 | Counter Staining Reagent | 5ml | RTU | RT |
10 | Differentiation Reagent | 6ml | RTU | RT |
11 | Mounting Media | 5ml | RTU | RT |
12 | Control slide (Human pancreas) | 1 slide | RTU | RT |
13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
As DAB is a carcinogen, please take necessary precautions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.