Caspase 3 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rabbit pancreas tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) 1:300 followed by conjugation to the secondary antibody and DAB staining

Rat lung lysate 30ug, probed (bs-0081R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD

$Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining$

Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 3 Polyclonal Antibody (bs-0081R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

$Lane 1: Mouse Lung lysates; Lane 2: Human Hela cell lysates; Lane 3: Mouse NIH\/3T3 cell lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Lung lysates; Lane 2: Human Hela cell lysates; Lane 3: Mouse NIH\/3T3 cell lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human Raji cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Lung lysates; Lane 4: Rat Lung lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Raji cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Lung lysates; Lane 4: Rat Lung lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Mouse Cerebrum lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Spleen lysates; Lane 7: Rat Lung lysates; Lane 8: Rat Lymph node lysates; Lane 9: Rat Cerebrum lysates; Lane 10: Human Hela cell lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Mouse Cerebrum lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Spleen lysates; Lane 7: Rat Lung lysates; Lane 8: Rat Lymph node lysates; Lane 9: Rat Cerebrum lysates; Lane 10: Human Hela cell lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat
Bovine
Pig
Chicken
Sheep
Rabbit
Others
Monkey

Overview
Catalog #	bs-0081R
Product Name	Caspase 3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Caspase 3(Active)
Immunogen Range	1-100/277
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	836
Swiss Prot	P42574
Subcellular location	Cytoplasm
Synonyms	CPP32; SCA-1; CPP32B; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; CASP3
Background	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200