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Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa
Tissue: Mouse colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60053M

Catalog #1: bsm-60053M

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Trichostatin A (2\u03bcM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, N2a, BRL\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:500\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60066M

Catalog #1: bsm-60066M

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa
Tissue: Mouse colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60054M

Catalog #1: bsm-60054M

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+SBA (5mM, 24hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa
Tissue: Human liver cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60067M
Tissue: Rat liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60067M
Tissue: Mouse kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60067M

Catalog #1: bsm-60067M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

K562 cell lysate (20ug) probed with bsm-51033M ACOX1 (153CT43.1.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ACOX1 (153CT43.1.1) Monoclonal Antibody (bsm-51033M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51033M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431; Lane 2: HeLa lysate at 20 \u00b5g per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ACTA1 (337CT30.10.1) Monoclonal Antibody (bsm-51104M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: K562; Lane 2: CEM lysate at 20 \u00b5g per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51104M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

A431 cell lysate probed with ACTA2 (3F9) Monoclonal Antibody (bsm-33187M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:400 overnight at 4\u00b0C, followed by incubation with a secondary antibody, DAB and counter-staining.
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Small intestine lysates; Lane 6: Mouse NIH\/3T3 cell lysates; Lane 7: Rat Stomach lysates; Lane 8: Rat Uterus lysates; Lane 9: Human A549 cell lysates; Lane 10: Human Hela cell lysates; Lane 11: Human A431 cell lysates; Lane 12: Human HepG2 cell lysates probed with ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33187M

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33187M-BF350

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-33187M-BF405

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-33187M-BF488

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-33187M-BF555

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-33187M-BF594

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-33187M-BF647

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-33187M-BF680

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-33187M-BF750

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-33187M-APC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33187M-APC-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bsm-33187M-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33187M-APC-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33187M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33187M-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33187M-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33187M-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33187M-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33187M-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33187M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33187M-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33187M-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bsm-33187M-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33187M-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33187M-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33187M-PerCP

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33187M-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33187M-PerCP-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33187M-RBITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-34147M

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-34147M-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-34147M-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-70322M

Applications: WB, IHC, ICC

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bsm-70011M

Applications: WB, IHC, ICC

Reactivity: Human, Mouse, Rat, All

Conjugate: Unconjugated

LoVo lysates probed with Actin, alpha skeletal muscle Polyclonal Antibody, Unconjugated (bs-33308M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Actin, alpha skeletal muscle Monoclonal Antibody, Unconjugated (bsm-33308M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33308M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (bsm-33297M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (bsm-33297M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33297M

Applications: IHC-P, IHC-F, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33308M-BF350

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-33308M-BF405

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-33308M-BF488

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-33308M-BF555

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-33308M-BF594

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-33308M-BF647

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647