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Lane 1: Rat Ovary lysates; Lane 2:SH-SY5Y cell lysates; Lane 3:HepG2 cell lysates; Lane 4: A431 cell lysates probed with ACE2 Polyclonal Antibody, Unconjugated (bs-23027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: ACE2 Protein Overexpression E.coli lysates; Lane 2: Control group E.coli Lysates probed with ACE2 Polyclonal Antibody, Unconjugated (bs-23027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ACE2 Polyclonal Antibody(bs-23027R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-23027R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat, Bacteria

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ASC Polyclonal Antibody, Unconjugated (bs-6741R) at 1:800 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ASC Polyclonal Antibody, Unconjugated (bs-6741R) at 1:800 overnight at 4\u00b0C, DAB staining.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ASC Polyclonal Antibody(bs-6741R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ASC Polyclonal Antibody(bs-6741R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6741R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: Unconjugated

Catalog #1: bs-6741R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bs-6741R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bs-6741R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: AbBy Fluor® 488

Catalog #1: bs-6741R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: AbBy Fluor® 555

Catalog #1: bs-6741R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: AbBy Fluor® 594

Catalog #1: bs-6741R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: AbBy Fluor® 647

Catalog #1: bs-6741R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: AbBy Fluor® 680

Catalog #1: bs-6741R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: AbBy Fluor® 750

Catalog #1: bs-6741R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: APC

Catalog #1: bs-6741R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: APC-Cy5

Catalog #1: bs-6741R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: APC-Cy5.5

Catalog #1: bs-6741R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: APC-Cy7

Catalog #1: bs-6741R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: Biotin

Catalog #1: bs-6741R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: Cy3

Catalog #1: bs-6741R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: Cy5

Catalog #1: bs-6741R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: Cy5.5

Catalog #1: bs-6741R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: Cy7

Catalog #1: bs-6741R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: FITC

Catalog #1: bs-6741R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: HRP

Catalog #1: bs-6741R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: PE

Catalog #1: bs-6741R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: PE-Cy3

Catalog #1: bs-6741R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: PE-Cy5

Catalog #1: bs-6741R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: PE-Cy5.5

Catalog #1: bs-6741R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: PE-Cy7

Catalog #1: bs-6741R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: PerCP

Catalog #1: bs-6741R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: PerCP-Cy5.5

Catalog #1: bs-6741R-PerCP-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: PerCP-Cy7

Catalog #1: bs-6741R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Bacteria, Others

Conjugate: RBITC

Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. P

Catalog #1: bs-0032R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: Unconjugated

Catalog #1: bs-0032R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0032R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0032R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0032R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0032R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0032R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0032R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0032R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0032R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: APC

Catalog #1: bs-0032R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: APC-Cy5

Catalog #1: bs-0032R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: APC-Cy5.5

Catalog #1: bs-0032R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: APC-Cy7

Catalog #1: bs-0032R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: Biotin

Catalog #1: bs-0032R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: Cy3

Catalog #1: bs-0032R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: Cy5

Catalog #1: bs-0032R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: Cy5.5

Catalog #1: bs-0032R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria

Conjugate: Cy7